Symposium report: emerging threats for human health - impact of socioeconomic and climate change on zooanthroponosis in the Republic of Sakha (Yakutia), Russia.

Population growth, socio-cultural and economic changes as well as technological progress have an immediate impact on the environment and human health in particular. Our steadily rising needs of resources increase the pressure on the environment and narrow down untainted habitats for plants and wild animals. Balance and resilience of ecosystems are further threatened by climate change, as temperature and seasonal shifts increase the pressure for all species to find successful survival strategies. Arctic and subarctic regions are especially vulnerable to climate change, as thawing of permafrost significantly transforms soil structures, vegetation and habitats. With rising temperature, the risk of zoonotic diseases in the Republic of Sakha (Yakutia) has also increased. As vegetation periods prolong and habitats broaden, zoonotic pathogens and their vectors find more favourable living conditions. Moreover, permafrost degradation may expose historic burial grounds and allow for reviving the vectors of deadly infections from the past. To assess the current state of knowledge and emerging risks in the light of the "One Health" concept, a German-Russian Symposium took place on 13 August 2018 in Yakutsk, Russian Federation. This symposium report presents the main findings generated from presentations and discussions.

Recent dynamics of hydro-ecosystems in thermokarst depressions in Central Siberia from satellite and in situ observations: Importance for agriculture and human life.

Alases, which are thermokarst depressions that are occupied by grasslands and lakes, are an important element of the Central Yakutian periglacial landscape. In recent decades, climatic changes in Central Yakutia have resulted in important changes in environmental conditions. We use different remote-sensing instruments (Landsat 8, TerraSAR-X, ENVISAT-RA2, and Jason-2) alongside in situ observations to investigate 1) the spatial distribution and water regime of alas lakes and their relationships with climatic and geomorphologic factors, 2) the relationship of the alas' grassland productivity with the water regime and 3) the potential of alas grasslands for local agriculture. During the 2002-2010 period, the lake water level rose by 1.3m on average, resulting in lake expansion throughout the region. Since 2011, the lake area decreased and the water level declined by 70cm on the middle terraces (low ground-ice content), while the wetting trend continued until 2016 at higher elevations. Small thermokarst lakes (<0.025km2), which indicate regions of young thermokarst, comprise up to 11% of the total lake area and experience high (30%) seasonal variations. In situ observations of the grassland dynamics show their synchronous cyclic variability with the lake extent and a general increasing trend for their productivity since 1985. Around 50% of these dynamics can be explained by the amount of pre-winter precipitation with a delay of two years. We explain this delay through the buffering effect of watershed soils. The cyclic variability of alas hydro-ecosystems strongly affects the local agriculture, which is based on horse and cattle breeding. We estimate that these alas grasslands can provide enough forage supply for local communities. However, the real alas yield is several times less than the theoretical value because of grassland degradation that is caused by recent thermokarst and waterlogging in the most productive phytocenosis.

High rate of N2 fixation by East Siberian cryophilic soil bacteria as determined by measuring acetylene reduction in nitrogen-poor medium solidified with gellan gum.

For evaluating N(2) fixation of diazotrophic bacteria, nitrogen-poor liquid media supplemented with at least 0.5% sugar and 0.2% agar are widely used for acetylene reduction assays. In such a soft gel medium, however, many N(2)-fixing soil bacteria generally show only trace acetylene reduction activity. Here, we report that use of a N(2) fixation medium solidified with gellan gum instead of agar promoted growth of some gellan-preferring soil bacteria. In a soft gel medium solidified with 0.3% gellan gum under appropriate culture conditions, bacterial microbiota from boreal forest bed soils and some free-living N(2)-fixing soil bacteria isolated from the microbiota exhibited 10- to 200-fold-higher acetylene reduction than those cultured in 0.2% agar medium. To determine the N(2) fixation-activating mechanism of gellan gum medium, qualitative differences in the colony-forming bacterial components from tested soil microbiota were investigated in plate cultures solidified with either agar or gellan gum for use with modified Winogradsky's medium. On 1.5% agar plates, apparently cryophilic bacterial microbiota showed strictly distinguishable microbiota according to the depth of soil in samples from an eastern Siberian Taiga forest bed. Some pure cultures of proteobacteria, such as Pseudomonas fluorescens and Burkholderia xenovorans, showed remarkable acetylene reduction. On plates solidified with 1.0% gellan gum, some soil bacteria, including Luteibacter sp., Janthinobacterium sp., Paenibacillus sp., and Arthrobacter sp., uniquely grew that had not grown in the presence of the same inoculants on agar plates. In contrast, Pseudomonas spp. and Burkholderia spp. were apparent only as minor colonies on the gellan gum plates. Moreover, only gellan gum plates allowed some bacteria, particularly those isolated from the shallow organic soil layer, to actively swarm. In consequence, gellan gum is a useful gel matrix to bring out growth potential capabilities of many soil diazotrophs and their consortia in communities of soil bacteria.